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Nat Methods. 2010 Nov;7(11):901-3. doi: 10.1038/nmeth.1515. Epub 2010 Oct 10.

Chemical synthesis of the mouse mitochondrial genome.

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  • 1The J. Craig Venter Institute, Rockville, Maryland, USA. dgibson@jcvi.org

Abstract

We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

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PMID:
20935651
[PubMed - indexed for MEDLINE]
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