Nat Methods. 2010 Nov;7(11):901-3. Epub 2010 Oct 10.

Chemical synthesis of the mouse mitochondrial genome.

Gibson DG, Smith HO, Hutchison CA 3rd, Venter JC, Merryman C.

Source

The J. Craig Venter Institute, Rockville, Maryland, USA. dgibson@jcvi.org

Abstract

We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

Comment in

Nat Methods. 2010 Nov;7(11):887-9.

PMID:: 20935651; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

DNA, Mitochondrial

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