Secondary cataract material collected with a glass cannula. A SEM study

Doc Ophthalmol. 1990 Oct;75(3-4):359-64. doi: 10.1007/BF00164851.

Abstract

Secondary cataract material from three patients, collected with a glass cannula approx. 18, 24 and 30 months respectively after operation, was prepared for SEM examination. For the soft samples this was done by filtration through a millipore filter followed by fixation and drying. The more solid material was suspended in a fixation solution, followed by centrifuging, suspension in 70% ethanol and drying on a specimen-holder. The short residence samples (18 months) showed mainly erythrocytes, some (inflammatory) cells and degenerated lens-fibre material. Most of the more solid material, which was collected more than 20 months after operation, showed fragments of (regenerated) capsule epithelium and pieces of solid lens-fibre material with fragments of capsule epithelium attached.

MeSH terms

  • Cataract / pathology*
  • Cataract Extraction
  • Humans
  • Lens, Crystalline / ultrastructure*
  • Time Factors