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Curr Opin Microbiol. 2010 Oct;13(5):619-24. doi: 10.1016/j.mib.2010.09.009. Epub 2010 Sep 29.

Studying bacterial transcriptomes using RNA-seq.

Author information

  • 1The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, Cambridgeshire, CB10 1SA, UK.

Abstract

Genome-wide studies of bacterial gene expression are shifting from microarray technology to second generation sequencing platforms. RNA-seq has a number of advantages over hybridization-based techniques, such as annotation-independent detection of transcription, improved sensitivity and increased dynamic range. Early studies have uncovered a wealth of novel coding sequences and non-coding RNA, and are revealing a transcriptional landscape that increasingly mirrors that of eukaryotes. Already basic RNA-seq protocols have been improved and adapted to looking at particular aspects of RNA biology, often with an emphasis on non-coding RNAs, and further refinements to current techniques will improve our understanding of gene expression, and genome content, in the future.

Copyright © 2010 Elsevier Ltd. All rights reserved.

PMID:
20888288
[PubMed - indexed for MEDLINE]
PMCID:
PMC3025319
Free PMC Article
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