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Cell. 2010 Oct 1;143(1):46-58. doi: 10.1016/j.cell.2010.09.001.

Long noncoding RNAs with enhancer-like function in human cells.

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  • 1The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.

Abstract

While the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian transcriptome, their biological functions has remained elusive. A few long ncRNAs that have been studied in any detail silence gene expression in processes such as X-inactivation and imprinting. We used a GENCODE annotation of the human genome to characterize over a thousand long ncRNAs that are expressed in multiple cell lines. Unexpectedly, we found an enhancer-like function for a set of these long ncRNAs in human cell lines. Depletion of a number of ncRNAs led to decreased expression of their neighboring protein-coding genes, including the master regulator of hematopoiesis, SCL (also called TAL1), Snai1 and Snai2. Using heterologous transcription assays we demonstrated a requirement for the ncRNAs in activation of gene expression. These results reveal an unanticipated role for a class of long ncRNAs in activation of critical regulators of development and differentiation.

Copyright © 2010 Elsevier Inc. All rights reserved.

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PMID:
20887892
[PubMed - indexed for MEDLINE]
PMCID:
PMC4108080
Free PMC Article

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