A: Experimental design for BPA pharmacokinetic study. B: Total BPA (open squares) and free BPA (closed squares) levels (ng/ml) in PND 3 male rats at 0.5, 1 and 2 hrs following s.c. injection of a BPA-oil depot at 10 μg/kg BW. C: Total BPA (open circles) and free BPA (closed circles) levels (ng/ml) in PND 3 male rats at 0.5, 1 and 2 hrs following oral ingestion of BPA in oil at 10 μg/kg BW. D: Comparison of free BPA levels (ng/ml) during first 2 hrs after s.c. injection (solid squares) and oral ingestion (solid circles) of BPA at dose of at 10 μg/kg BW. * denotes p < 0.05 for 0.5 hr values between injection versus oral exposure. E: Comparison of total BPA levels (ng/ml) during first 2 hrs after s.c. injection (open squares) and oral ingestion (open circles) of BPA at dose of at 10 μg/kg BW.

A: Experimental design to compare prostate response to 16 weeks of T+E treatment as adults in rats exposed neonatally to oil, oral BPA (10 μg/kg BW) or s.c. injection of BPA (10 μg/kg BW) on PND 1, 3 and 5. B: PIN scores in the ventral (VP), lateral (LP) and dorsal (DP) prostate lobes collected on day 200 of life in the three neonatal treatment groups. * denotes p < 0.05 versus oil control for the separate prostate lobes.

Prostate lobes of day 200 rats treated neonatally with oil (A-B), oral BPA at 10 μg/kgBW (B-C, I) or s.c. injection of BPA at 10 μg/kgBW (E-G) plus T+E as adults. A: Normal histologic region observed in LP lobe of rat treated with neonatal oil and adult T+E showing normal ductal architecture and intraluminal secretions. B: Normal histologic region found in VP lobe of rat treated with neonatal oil and adult T+E shows a single cell layer of columnar epithelium and limited stromal tissue. C: LP lobe from rat given neonatal oral BPA and adult T+E exhibiting high-grade PIN. Arrows point to some of the nuclei with prominent nucleoli which is seen throughout the acinus. Note variable nuclear size and shape and aberrant piling as compared to normal epithelium in adjacent acini in upper left. D: VP lobe from rat given neonatal oral BPA and adult T+E exhibiting adenoma (arrow) and atypical hyperplasia (arrowhead) within crowded acini. E: LP lobe from rat given neonatal s.c. BPA plus adult T+E exhibiting high-grade PIN similar (arrows) and a thickened stromal cell compartment. F: VP lobe from rat given neonatal s.c. BPA and adult T+E showing adenoma ((arrows) and epithelial hyperplasia. G: LP lobe from rat given neonatal s.c. BPA plus adult T+E contains inflammatory cell infiltration. Solid arrows point to intraluminal leukocytes, arrowhead marks stromal lymphocytes and dashed arrow identifies proliferative reaction in the epithelial layer. H: LP lobe from rat given neonatal oral BPA plus adult T+E contains acini with intraluminal leukocytes (arrowhead) and epithelial piling (arrow) characteristic of reactive proliferation to the immune cells. Bar denotes 50 μm in A, B and G, 40 μm in C-F, H and insets are at 100× magnification.