Biochimie. 2011 Feb;93(2):141-8. doi: 10.1016/j.biochi.2010.08.014. Epub 2010 Sep 22.

Novel cathepsin L-like protease from dermestid beetle Dermestes frischii maggot.

Papisova AI, Javadov A, Rudenskaya YA, Balandina GN, Zhantiev RD, Korsunovskaia OS, Dunaevsky YE, Rudenskaya GN.

Source

Department of Chemistry, Moscow State University, Moscow 119992, Russia. laboratoriahps@hotmail.com

Abstract

A novel cathepsin L-like protease from dermestid beetle Dermestes frischii maggot guts was obtained and investigated. The protease was isolated through affinity chromatography at arginine-diasorb followed by FPLC gel-filtration at Superdex 75. Protease is active against chromogenic peptide substrates, containing Arg or Leu in P1 position and a hydrophobic residue in P2 position. PH optimum is about 4,5 and temperature optimum at 40°C. Enzyme is inhibited completely by HgCl(2) and leupeptin that prove it's belonging to cysteine proteases of papain family. cDNA analysis of cathepsin L-like protease showed that protein sequence consists of 339 amino acid residues. Mature cysteine protease contains 219 amino acid residues corresponding to molecular mass 24027.20Da. Residues of the active site were identified: Gln(140), Cys(146), His(285), Asn(306) and Trp(308). Calculated pI is 4,73. The amino acid sequence of the cystein protease from dermestid beetle displays high structural homology with cathepsin L of other insects.

PMID:: 20868724; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Save items

Related citations in PubMed

Molecular cloning of a multidomain cysteine protease and protease inhibitor precursor gene from the tobacco hornworm (Manduca sexta) and functional expression of the cathepsin F-like cysteine protease domain. [Insect Biochem Mol Biol. 2010]
Molecular cloning of a multidomain cysteine protease and protease inhibitor precursor gene from the tobacco hornworm (Manduca sexta) and functional expression of the cathepsin F-like cysteine protease domain.
Miyaji T, Murayama S, Kouzuma Y, Kimura N, Kanost MR, Kramer KJ, Yonekura M. Insect Biochem Mol Biol. 2010 Dec; 40(12):835-46. Epub 2010 Aug 18.
Cloning and expression of the cathepsin F-like cysteine protease gene in Escherichia coli and its characterization. [J Microbiol. 2007]
Cloning and expression of the cathepsin F-like cysteine protease gene in Escherichia coli and its characterization.
Joo HS, Koo KB, Park KI, Bae SH, Yun JW, Chang CS, Choi JW. J Microbiol. 2007 Apr; 45(2):158-67.
Characterisation of Tc-cpl-1, a cathepsin L-like cysteine protease from Toxocara canis infective larvae. [Mol Biochem Parasitol. 1998]
Characterisation of Tc-cpl-1, a cathepsin L-like cysteine protease from Toxocara canis infective larvae.
Loukas A, Selzer PM, Maizels RM. Mol Biochem Parasitol. 1998 May 1; 92(2):275-89.
Identification and characterization of a cathepsin L-like cysteine protease from Gnathostoma spinigerum. [Mol Biochem Parasitol. 2008]
Identification and characterization of a cathepsin L-like cysteine protease from Gnathostoma spinigerum.
Kongkerd N, Uparanukraw P, Morakote N, Sajid M, McKerrow JH. Mol Biochem Parasitol. 2008 Aug; 160(2):129-37. Epub 2008 May 8.
The novel bovine serpin endopin 2C demonstrates selective inhibition of the cysteine protease cathepsin L compared to the serine protease elastase, in cross-class inhibition. [Biochemistry. 2005]
The novel bovine serpin endopin 2C demonstrates selective inhibition of the cysteine protease cathepsin L compared to the serine protease elastase, in cross-class inhibition.
Hwang SR, Stoka V, Turk V, Hook VY. Biochemistry. 2005 May 31; 44(21):7757-67.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.

Recent activity

Clear Turn Off Turn On

Novel cathepsin L-like protease from dermestid beetle Dermestes frischii maggot.
Novel cathepsin L-like protease from dermestid beetle Dermestes frischii maggot.
Biochimie. 2011 Feb ;93(2):141-8. doi: 10.1016/j.biochi.2010.08.014. Epub 2010 Sep 22 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: