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Immunity. 2010 Sep 24;33(3):412-23. doi: 10.1016/j.immuni.2010.08.016.

Visualizing the functional diversification of CD8+ T cell responses in lymph nodes.

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  • 1Institut Pasteur, Unité des Dynamiques des Réponses Immunes, Département d'Immunologie, Paris, France.

Abstract

CD8(+) T cell responses generate effector cells endowed with distinct functional potentials but the contribution of early events in this process is unclear. Here, we have imaged T cells expressing a fluorescent reporter for the activation of the interferon-γ (IFN-γ) locus during priming in lymph nodes. We have demonstrated marked differences in the efficiency of gene activation during stable T cell-dentritic cell (DC) contacts, influenced in part by signal strength. Imaging the first cell division, we have demonstrated that heterogeneity in T cell functional potential was largely apparent as T cells initiated clonal expansion. Moreover, by analyzing the fate of single activated T cells ex vivo, we have provided evidence that these early differences resulted in clonal progenies with distinct functional properties. Thus, the early set of T cell-DC interactions in lymph nodes largely contribute to the heterogeneity of T cell responses through the generation of functionally divergent clonal progenies.

Copyright © 2010 Elsevier Inc. All rights reserved.

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PMID:
20850354
[PubMed - indexed for MEDLINE]
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