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Cell. 2010 Sep 17;142(6):868-78. doi: 10.1016/j.cell.2010.08.013.

Regulation of replication termination by Reb1 protein-mediated action at a distance.

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  • 1Department of Molecular Biology and Biochemistry, Medical University of South Carolina, Charleston, SC 29425, USA.


DNA transactions driven by long-range protein-mediated inter- and intrachromosomal interactions have been reported to influence gene expression. Here, we report that site-specific replication termination in Schizosaccharomyces pombe is modulated by protein-mediated interactions between pairs of Ter sites located either on the same or on different chromosomes. The dimeric Reb1 protein catalyzes termination and mediates interaction between Ter sites. The Reb1-dependent interactions between two antiparallel Ter sites in cis caused looping out of the intervening DNA in vitro and enhancement of fork arrest in vivo. A Ter site on chromosome 2 interacted pairwise with two Ter sites located on chromosome 1 by chromosome kissing. Mutational inactivation of the major interacting Ter site on chromosome 1 significantly reduced fork arrest at the Ter site on chromosome 2, thereby revealing a cooperative mechanism of control of replication termination.

Copyright © 2010 Elsevier Inc. All rights reserved.

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