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Mol Imaging Biol. 2011 Oct;13(5):874-85. doi: 10.1007/s11307-010-0416-8.

Imaging the bio-distribution of fluorescent probes using multispectral epi-illumination cryoslicing imaging.

Author information

  • 1Chair for Biological Imaging, Technische Universit√§t M√ľnchen, Munich, Germany.

Abstract

PURPOSE:

The increasing availability of fluorescent probes for in vivo optical imaging enables the interrogation of complex biological processes in small animals serving as models for human-like tissue function and disease. However, the validation of probe bio-distribution during their development or the study of different disease models, in support of in vivo imaging studies, is not straightforward.

PROCEDURES:

The imaging system developed consists of a customized multispectral planar imager that has been adapted on a commercial cryomicrotome and provides a powerful modality for ex vivo imaging of small animals.

RESULTS:

The ability to capture 3D anatomical (color) and fluorescence volumetric distributions of multiple fluorescent markers in high resolution is showcased.

CONCLUSIONS:

Serving both as a method for accurately imaging the bio-distribution of multiple fluorescent agents inside organisms and as a modality for the validation of non-invasive methods, multispectral cryoslicing imaging offers useful insights to ex vivo optical imaging of molecular probes.

PMID:
20838910
[PubMed - indexed for MEDLINE]
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