(A) Gross and (B, C) cross sectional H&E histology of lungs isolated from CAG-miR-21;K-ras^LA2 mice and K-ras^LA2 littermate controls at 18 weeks of age. (C) Magnification of boxed lesions in (B). Arrows in top and bottom panels indicate a hyperplastic lesion and adenoma, respectively. Scale bars, (B) 5 mm, (C) 200 μm.
(D) Nodules grossly visible were counted on the lung surface of CAG-miR-21;K-ras^LA2 (n = 5) and K-ras^LA2 (n = 4) mice sacrificed at 18 weeks of age. Results are mean ± SEM. P value 0.013 using two-tailed, unpaired Student’s t-test.
(E) Tumor burden measured as the ratio of total tumor area to total lung area of the lungs counted in (D). Results are mean ± SEM. P value 0.045 using two-tailed, unpaired Student’s t-test.
(F) Quantification of tumor lesions on lung H&E cross-section from CAG-miR-21;K-ras^LA2 (n = 49) and K-ras^LA2 (n = 40) mice over time. Slopes were determined by linear regression, P value 0.0027.
(G) Quantification of proliferating cells detected by Ki67 antibody immunostaining of lung tumors. Results are mean ± SEM (n = 12). P value 0.01 by two-tailed, unpaired Student’s t-test.
(H) Survival curve of CAG-miR-21;K-ras^LA2 (n = 27) and K-ras^LA2 (n = 32) mice (P = 0.367) with median survival of 270 and 203 days, respectively. WT (n = 10), CAG-miR-21 (n = 20).
(I) Tumor spectra in CAG-miR-21;K-ras^LA2 (n = 27) and K-ras^LA2 mice (n = 32).
(J) Lung H&E cross sections from mice in the survival cohort were analyzed and all lesions were classified for tumor grade. CAG-miR-21;K-ras^LA2 (n = 27), K-ras^LA2 (n = 32). Hyperplasia, P value < 0.0001. Atypical adenomatous hyperplasia, P value 0.017. Adenoma, P value < 0.0001. Adenoma with atypia, P value 0.0042. Adenocarcinoma, P value 0.033. All data represented as the mean ± SEM of the number of lesions per lung. (K) Rate of conversion of adenoma to adenocarcinoma, data represented as mean ± SEM, P value 0.83. See also Figure S1.

(A) Luciferase activity in COS cells co-transfected with miR-21 or control vector and miR-21 responsive target gene 3′ UTRs or miR-21 site mutant 3′ UTR luciferase reporters. Data represented as mean ± SEM and normalized to empty vector (no miR-21) control. n = 4.
(B) Luciferase activity in COS cells cotransfected with miR-21 or empty vector and indicated miR-21 unresponsive 3′ UTRs. Data represented as mean ± SEM and normalized to empty vector (no miR-21) control. n = 4.

(B) Quantification of TUNEL positive nuclei per lung tumor. K-ras^LA2 (n = 16) and CAG-miR-21;K-ras^LA2 (n = 62). Results are mean ± SEM. P value 0.007 by unpaired, two-tailed Student’s t-test.
(C) Quantification of TUNEL staining in miR-21^−/−;K-ras^LA2 and K-ras^LA2 controls. K-ras^LA2 (n = 42) and miR-21^−/−;K-ras^LA2 (n = 22). Results are mean ± SEM. P value 0.28 using unpaired, two-tailed Student’s t-test.
(D) Western blot analysis of lung lysates from wild-type (WT) and CAG-miR-21 mice and six isolated lung tumors from CAG-miR-21;K-ras^LA2 and K-ras^LA2 mice. Antibodies used are shown on right.
(E) Western blot analysis of lung lysates from WT and miR-21^−/− mice and six isolated lung tumors from miR-21^−/−;K-ras^LA2 and K-rs^LA2 mice. Antibodies are shown on right. (F) Quantification of protein expression from Western blots shown in Figure 4C, Figure 5D, and Figure S3A and S3B. Bar values indicate the mean ± SEM of protein levels in six independent tumors from CAG-miR-21;K-ras^LA2 mice after normalization to Gapdh and represented as fraction of the protein levels in the K-ras^LA2 controls. Dashed line indicates the protein level in K-ras^LA2 tumors.

Proposed model showing that miR-21 potentiates oncogenic Ras signaling and attenuates apoptosis through repression of multiple tumor suppressors, miR-21 target genes.

(A) Gross and (B) cross sectional H&E histology of lungs isolated from miR-21^−/−;K-ras^LA2 and K-ras^LA2 mice at 20 weeks of age. Scale bar, 5 mm.
(C) Quantification of nodules grossly visible on the lung surface of miR-21^−/minus;;K-ras^LA2 (n = 5) and K-ras^LA2 (n = 3) mice at 20 weeks of age. Results are mean ± SEM. P value 0.008 using two-tailed, unpaired Student’s t-test.
(D) Tumor burden measured as the ratio of total tumor area to total lung area of the lungs counted in (C). Results are mean ± SEM. P value 0.015 using two-tailed, unpaired Student’s t-test.
(E) Quantification of proliferating cells detected by Ki67 antibody immunostaining of lung tumors. Results are mean + SEM (n = 6). P value 0.4 by two-tailed, unpaired Student’s t-test.
(F) Tumor spectra in K-ras^LA2 and miR-21^−/−;K-ras^LA2 mice.
(G) Lung H&E cross sections from miR-21^−/−;K-ras^LA2 (n = 14), K-ras^LA2 (n = 6) mice were analyzed and all lesions were classified for tumor grade. Hyperplasia, P value 0.023. Adenoma, P value 0.057. All data represented as the mean ± SEM of the number of lesions per lung.
(H) Rate of conversion of hyperplasia to adenoma. Data represented as mean ± SEM, P value 0.089. See also Figure S2.

(A) MiR-21 expression in lung tumors from CAG-miR-21;K-ras^LA2 and K-ras^LA2 mice (after K-ras activation) compared to normal lung from wild-type, K-ras^LA2 (prior to K-ras activation) and CAG-miR-21 by realtime PCR. Results are mean ± SEM.
(B) Northern blot analysis of miR-21 expression in lung tumors from CAG-miR-21;K-ras^LA2 and K-ras^LA2 mice (after K-ras activation) compared to normal lung from wild-type, K-ras^LA2 (prior to K-ras activation) and CAG-miR-21.
(C) Western blot analysis of lung lysates from normal lung from wild-type (WT) and CAG-miR-21 mice and six isolated lung tumors from CAG-miR-21;K-ras^LA2 and K-ras^LA2 mice. Antibodies used are shown on right.
(D) Western blot analysis of lung lysates from wild-type (WT) and miR-21^−/− mice and six isolated tumors from miR-21^−/−;K-ras^LA2 and K-ras^LA2 mice. Antibodies used are shown on right. See also Figure S3.

(A) Western blot analysis (left panel) for cleaved caspase 3 in protein lysates from wild-type and miR-21^−/− MEFs immortalized with T-Ag, transformed with H-rasV12, and treated with increasing concentrations of doxorubicin. Antibodies used are shown on right. Quantification of cleaved caspase 3 levels normalized to pro-caspase 3 and represented relative to untreated wild-type MEFs (right panel).
(B) Western blot analysis (left panel) for cleaved caspase 3 in protein lysates from miR-21^−/− MEFs immortalized with T-Ag, transformed with H-rasV12, and transduced with lentivirus expressing miR-21 (lenti-miR-21) or empty vector, and treated with increasing concentrations of doxorubicin. Antibodies used are shown on right. Quantification of cleaved caspase 3 levels normalized to pro-caspase 3 and represented relative to untreated wild-type MEFs (right panel).
(C) TUNEL assay of wild-type and miR-21^−/− MEFs treated with vehicle or 1 μM doxorubicin for 12 hours. Data represented as mean ± SEM, n = 6. See also Figure S4.