FEBS Lett. 2010 Oct 8;584(19):4138-44. doi: 10.1016/j.febslet.2010.08.053. Epub 2010 Sep 7.

Interplay between the cpSRP pathway components, the substrate LHCP and the translocase Alb3: an in vivo and in vitro study.

Bals T, Dünschede B, Funke S, Schünemann D.

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Molecular Biology of Plant Organelles, Ruhr-University Bochum, Bochum, Germany.

Abstract

The chloroplast signal recognition particle (cpSRP) and its receptor, cpFtsY, posttranslationally target the nuclear-encoded light-harvesting chlorophyll-binding proteins (LHCPs) to the translocase Alb3 in the thylakoid membrane. In this study, we analyzed the interplay between the cpSRP pathway components, the substrate protein LHCP and the translocase Alb3 by using in vivo and in vitro techniques. We propose that cpSRP43 is crucial for the binding of LHCP-loaded cpSRP and cpFtsY to Alb3. In addition, our data suggest that a direct interaction between Alb3 and LHCP contributes to the formation of this complex.

PMID:: 20828566; [PubMed - indexed for MEDLINE]

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Non-identical contributions of two membrane-bound cpSRP components, cpFtsY and Alb3, to thylakoid biogenesis. [Plant J. 2008]
Non-identical contributions of two membrane-bound cpSRP components, cpFtsY and Alb3, to thylakoid biogenesis.
Asakura Y, Kikuchi S, Nakai M. Plant J. 2008 Dec; 56(6):1007-17. Epub 2008 Aug 21.
Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate not required. [J Cell Biol. 2003]
Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate not required.
Moore M, Goforth RL, Mori H, Henry R. J Cell Biol. 2003 Sep 29; 162(7):1245-54.
Canonical signal recognition particle components can be bypassed for posttranslational protein targeting in chloroplasts. [Plant Cell. 2007]
Canonical signal recognition particle components can be bypassed for posttranslational protein targeting in chloroplasts.
Tzvetkova-Chevolleau T, Hutin C, Noël LD, Goforth R, Carde JP, Caffarri S, Sinning I, Groves M, Teulon JM, Hoffman NE, et al. Plant Cell. 2007 May; 19(5):1635-48. Epub 2007 May 18.
Review Component interactions, regulation and mechanisms of chloroplast signal recognition particle-dependent protein transport. [Eur J Cell Biol. 2010]
Review Component interactions, regulation and mechanisms of chloroplast signal recognition particle-dependent protein transport.
Richter CV, Bals T, Schünemann D. Eur J Cell Biol. 2010 Dec; 89(12):965-73. Epub 2010 Aug 14.
Review Structure and function of the chloroplast signal recognition particle. [Curr Genet. 2004]
Review Structure and function of the chloroplast signal recognition particle.
Schünemann D. Curr Genet. 2004 Jan; 44(6):295-304. Epub 2003 Oct 21.

See reviews... See all...

Cited by 3 PubMed Central articles

Truncated photosystem chlorophyll antenna size in the green microalga Chlamydomonas reinhardtii upon deletion of the TLA3-CpSRP43 gene. [Plant Physiol. 2012]
Truncated photosystem chlorophyll antenna size in the green microalga Chlamydomonas reinhardtii upon deletion of the TLA3-CpSRP43 gene.
Kirst H, Garcia-Cerdan JG, Zurbriggen A, Ruehle T, Melis A. Plant Physiol. 2012 Dec; 160(4):2251-60. Epub 2012 Oct 5.
Assembly of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii requires expression of the TLA2-CpFTSY gene. [Plant Physiol. 2012]
Assembly of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii requires expression of the TLA2-CpFTSY gene.
Kirst H, García-Cerdán JG, Zurbriggen A, Melis A. Plant Physiol. 2012 Feb; 158(2):930-45. Epub 2011 Nov 23.
Interaction studies between the chloroplast signal recognition particle subunit cpSRP43 and the full-length translocase Alb3 reveal a membrane-embedded binding region in Alb3 protein. [J Biol Chem. 2011]
Interaction studies between the chloroplast signal recognition particle subunit cpSRP43 and the full-length translocase Alb3 reveal a membrane-embedded binding region in Alb3 protein.
Dünschede B, Bals T, Funke S, Schünemann D. J Biol Chem. 2011 Oct 7; 286(40):35187-95. Epub 2011 Aug 8.

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Interplay between the cpSRP pathway components, the substrate LHCP and the trans...
Interplay between the cpSRP pathway components, the substrate LHCP and the translocase Alb3: an in vivo and in vitro study.
FEBS Lett. 2010 Oct 8 ;584(19):4138-44. doi: 10.1016/j.febslet.2010.08.053. Epub 2010 Sep 7 .

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