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Korean J Physiol Pharmacol. 2010 Aug;14(4):205-12. doi: 10.4196/kjpp.2010.14.4.205. Epub 2010 Aug 31.

Proteomic analysis of differentially expressed proteins in bovine endometrium with endometritis.

Author information

  • 1Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon 590-832, Korea.

Abstract

Endometritis is one of the primary reasons for reproductive failure. In order to investigate endometritis-associated marker proteins, proteomic analysis was performed on bovine endometrium with endometritis. In bovine endometritis, desmin, α-actin-2, heat-shock protein (HSP) 27, peroxiredoxin-6, luteinizing hormone receptor isoform 1, collectin-43 precursor, deoxyribonuclease-I (DNase-I), and MHC class I heavy chain (MHC-Ih) were up-regulated. In contrast, transferrin, interleukin-2 precursor, hemoglobin β subunit, and potassium channel tetramerisation domain-containing 11 (KCTD11) were down-regulated in comparison to normal endometrium. The proteomic results were validated by semiquantitative-PCR and immunoblot analysis. The mRNA levels of desmin, transferrin, α-actin-2, HSP27, KCTD11, and MHC-Ih were up-regulated by over 1.5-fold, and showed a pattern similar to their proteomic profiles. Desmin and α-actin-2 protein showed positive correlations between proteomic analysis and immunoblot analysis. These results suggest that desmin and α-actin-2 may play important roles in endometritis-related function, and could be useful markers for the diagnosis of bovine endometritis.

KEYWORDS:

Actin; Desmin; Endometritis; Proteomics

PMID:
20827334
[PubMed]
PMCID:
PMC2933436
Free PMC Article
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