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Nan Fang Yi Ke Da Xue Xue Bao. 2010 Aug;30(8):1810-2, 1816.

[Effects of radiosensitivity and X-ray dose on miR-7 expression in nasopharyngeal carcinoma].

[Article in Chinese]

Author information

  • 1Department of Radiotherapy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. flashido@yahoo.com.cn

Abstract

OBJECTIVE:

To investigate the effects of radiosensitivity and X-ray dose on the expression of miR-7 in nasopharyngeal carcinoma (NPC) cells.

METHODS:

Low radiosensitive NPC cells CNE-1 and high radiosensitive NPC cells CNE-2 were exposed to 0, 2 and 8 Gy X-ray. The total RNAs of the cell lines were extracted 10 h after radiation for reverse transcription of miR-7 and 18S rRNA by stem-loop primer and random hexamers, respectively. The non-irradiated CNE-1 cells served as the control sample and the relative quantity of the expression level was calculated after real-time PCR using SyBR green.

RESULTS:

miR-7 expression differed significantly between CNE-1 and CNE-2 cells (4.49-/+3.62 vs 1.29-/+1.10, F=135.483, P<0.001). The radiation dose also significantly affected the expression of miR-7 in NPC cells (F=39.565, P<0.001). CNE-1 cells with a 2 Gy exposure had the highest expression level of miR-7, while the non-irradiated CNE-1 cells had the lowest expression. CNE-2 cells exposed to 2 Gy X-ray had the lowest expression level of miR-7 and the non-irradiated CNE-2 cells had the highest.

CONCLUSION:

Radiosensitivity and radiation dose of X-ray have significant effect on the expression of miR-7 in NPC cells, indicating that miR-7 plays an important role in radioresistance of NPC cells to X-ray, and suppressed miR-7 expression may elevate the radiosensitivity of NPC cells.

PMID:
20813671
[PubMed - indexed for MEDLINE]
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