Targeting the reversibly oxidized protein tyrosine phosphatase superfamily

Sci Signal. 2010 Aug 31;3(137):pl2. doi: 10.1126/scisignal.3137pl2.

Abstract

Controlled production of reactive oxygen species leads to reversible oxidation of protein tyrosine phosphatases (PTPs) and has emerged as an important tier of regulation over phosphorylation-dependent signal transduction. We present a modified cysteinyl-labeling assay that detects reversible oxidation of members of each of the different PTP subclasses. Here, we describe the methods for enriching reversibly oxidized PTPs from complex protein extracts, illustrating the procedure in IMR90 fibroblasts.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Humans
  • Oxidation-Reduction
  • Phosphorylation
  • Protein Tyrosine Phosphatases / analysis*
  • Protein Tyrosine Phosphatases / chemistry*
  • Protein Tyrosine Phosphatases / metabolism
  • Reactive Oxygen Species / chemistry*
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / physiology

Substances

  • Reactive Oxygen Species
  • Protein Tyrosine Phosphatases