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Dev Dyn. 2010 Oct;239(10):2603-18. doi: 10.1002/dvdy.22393.

Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis.

Author information

  • 1Biological Sciences, Smith College, Northampton, Massachusetts 01063, USA. mbarresi@email.smith.edu

Abstract

The formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a "shelf-screen" to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were always associated with axon defects, supporting an important role for axon-glial interactions during axon scaffold development. The genes disrupted in these viral lines have all been identified, providing a powerful new resource for the study of axon guidance, glio- and neurogenesis, and neuron-glial interactions during development of the vertebrate CNS.

PMID:
20806318
[PubMed - indexed for MEDLINE]
PMCID:
PMC2967675
Free PMC Article

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