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    Chirality. 2011 Feb;23(2):128-32. doi: 10.1002/chir.20887. Epub 2010 Aug 27.

    Estimating chromatographic enantioselectivity (α) from gradient enantioselective chromatography data.

    Gong X, Craven T, Schafer W, Pirzada Z, Biba M, Welch CJ.

    Source

    Separation and Purification Center of Excellence, Merck & Co., Inc., Rahway, New Jersey, USA. Xiaoyi_Gong@Merck.Com

    Abstract

    Calculation of chromatographic enantioselectivity (α) is critically important in enantioselective chromatographic method development studies. The fact that α can only be calculated from isocratic elution conditions, whereas gradient elution conditions are predominantly used in method development screening, presents some problems for the use of α as a scoring indicator for automated, intelligent enantioselective chromatography method development systems. In this study, an empirical algorithm was developed to estimate α at isocratic conditions based upon information collected from a gradient elution. The algorithm was validated for SFC applications and has been shown to accurately predict enantioselectivity for a wide variety of racemic test analytes eluted on different chiral column and mobile phase conditions.

    Copyright © 2010 Wiley-Liss, Inc.

    PMID:
    20803492
    [PubMed - indexed for MEDLINE]

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