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Nat Med. 2010 Sep;16(9):1042-7. doi: 10.1038/nm.2205. Epub 2010 Aug 29.

Clinical microfluidics for neutrophil genomics and proteomics.

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  • 1Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. kkotz@partners.org

Abstract

Neutrophils have key roles in modulating the immune response. We present a robust methodology for rapidly isolating neutrophils directly from whole blood with 'on-chip' processing for mRNA and protein isolation for genomics and proteomics. We validate this device with an ex vivo stimulation experiment and by comparison with standard bulk isolation methodologies. Last, we implement this tool as part of a near-patient blood processing system within a multi-center clinical study of the immune response to severe trauma and burn injury. The preliminary results from a small cohort of subjects in our study and healthy controls show a unique time-dependent gene expression pattern clearly demonstrating the ability of this tool to discriminate temporal transcriptional events of neutrophils within a clinical setting.

PMID:
20802500
[PubMed - indexed for MEDLINE]
PMCID:
PMC3136804
Free PMC Article

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