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Curr Opin Struct Biol. 2010 Oct;20(5):640-8. doi: 10.1016/j.sbi.2010.07.006.

Cellular structural biology.

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  • 1Tokyo Metropolitan University, Department of Chemistry, Minami-Osawa, Hachioji, Tokyo 192-0397, Japan.

Abstract

While we appreciate the complexity of the intracellular environment as a general property of every living organism, we collectively lack the appropriate tools to analyze protein structures in a cellular context. In-cell NMR spectroscopy represents a novel biophysical tool to investigate the conformational and functional characteristics of biomolecules at the atomic level inside live cells. Here, we review recent in-cell NMR developments and provide an outlook towards future applications in prokaryotic and eukaryotic cells. We hope to thereby emphasize the usefulness of in-cell NMR techniques for cellular studies of complex biological processes and for structural analyses in native environments.

Copyright © 2010 Elsevier Ltd. All rights reserved.

PMID:
20801639
[PubMed - indexed for MEDLINE]
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