Carbohydr Res. 2010 Oct 13;345(15):2252-61. Epub 2010 Jul 25.

Structural characterization of glycoprotein NGAL, an early predictive biomarker for acute kidney injury.

Zhao C, Ozaeta P, Fishpaugh J, Rupprecht K, Workman R, Grenier F, Ramsay C.

Source

Diagnostic Research Analytical Chemistry, Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064, United States. cheng.zhao@abbott.com

Abstract

Neutrophil gelatinase-associated lipocalin (NGAL) is a promising new renal biomarker that can reduce the time to diagnose acute kidney injury (AKI). There is little information available about complex glycans on NGAL. Detailed structural characterization of NGAL is necessary to understand the structural variability of NGAL used as a standard in the NGAL immunoassay. This study demonstrated that 7-9% of mutant (C87S) recombinant NGAL was N-glycosylated and no O-glycosylation was detected. The NGAL sequence was confirmed by nanoLC/MS/MS following in gel and in solution trypsin digestion, and the N-glycosylation site was localized by MS/MS. Six different mutant recombinant NGAL samples (samples A-F) were analyzed in this study; however, these samples demonstrated two different glycan patterns. Forty-one N-glycans were detected in sample A and the more abundant N-glycans were unsialylated. Forty-three N-glycans were detected in sample F and the more abundant N-glycans were sialylated. Each of the other four samples (B-E) had a similar N-glycan pattern as sample F.

PMID:: 20800224; [PubMed - indexed for MEDLINE]

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MeSH Terms

Substances

Glycoproteins

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Chemical compound information

Glycoproteins

MW: 741.69 g/mol

MF: C₂₈H₄₇N₅O₁₈

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