A: Rainbow-colored ribbon drawing of PflBenF with a dot surface representation of the pore calculated using HOLE21 viewed parallel to the cell membrane.
B: Stereodrawing of PflBenF with the pore viewed from the periplasmic face of the protein (using the same color coding as in Fig. 1A).
C: Sequence alignment of PlfBenF (gi|123657525; PDB Code 3JTY),
P. putida BenF (gi|81441797),
P. aeruginosa OpdK porin (gi|81539679; PDB Code 2QTK),
P. putida outer membrane protein (gi|167033173),
Acinetobacter baumannii VanP (gi|193076825),
A. sp. RUH2654 OprD family porin (gi|260549927), and
P. aeruginosa D2 porin (gi|417518; PDB Code 2ODJ) generated using ClustalW23 and color coded with ESPRIPT24; numbers after “_” denote sequence identity with respect to the PflBenF and aromatic acid selectivity pore residues are marked with
.
D: Semitransparent representation of the solvent accessible periplasmic (upper panel) and extracellular (lower panel) surfaces of PlfBenF, color coded for qualitative electrostatic potential (generated using PyMol) shown with polypeptide chain as ribbon, using the same orientation as in Fig. 1B.