Display Settings:

Format

Send to:

Choose Destination
Mol Microbiol. 2010 Oct;78(2):331-43. doi: 10.1111/j.1365-2958.2010.07331.x. Epub 2010 Aug 29.

Temporal anatomy of an epigenetic switch in cell programming: the white-opaque transition of C. albicans.

Author information

  • 1Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA.

Abstract

The human pathogen Candida albicans undergoes a well-defined switch between two distinct cell types, named 'white' and 'opaque'. White and opaque cells differ in metabolic preferences, mating behaviours, cellular morphologies and host interactions. Each cell type is stable through many generations; switching between them is rare, stochastic and occurs without any known changes in the primary sequence of the genome; thus the switch is epigenetic. The white-opaque switch is regulated by a transcriptional circuit, composed of four regulators arranged in a series of interlocking feedback loops. To understand how switching occurs, we investigated the order of regulatory changes that occur during the switch from the opaque to the white cell type. Surprisingly, changes in key transcriptional regulators occur gradually, extending over several cell divisions with little cell-to-cell variation. Additional experiments, including perturbations to regulator concentrations, refine the signature of the commitment point. Transcriptome analysis reveals that opaque cells begin to globally resemble white cells well before they irreversibly commit to switching. We propose that these characteristics of the switching process permit C. albicans to 'test the waters' before making an all-or-none decision.

© 2010 Blackwell Publishing Ltd.

PMID:
20735781
[PubMed - indexed for MEDLINE]
PMCID:
PMC3057202
Free PMC Article

Images from this publication.See all images (6)Free text

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Blackwell Publishing Icon for PubMed Central
    Loading ...
    Write to the Help Desk