Andrologia. 1990 Sep-Oct;22(5):427-35.

Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test.

Source

Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.

Abstract

Monoclonal antibodies specific for acrosomal proteins were prepared and characterized. Two of these monoclonal antibodies, ACR.3 and ACR.11, reacted with the same molecular specificity of acrosomal antigens (18-20 kDa), antibody ACR.4 reacted with the 25-27 kDa antigens, and antibody ACR.2 reacted with several forms of boar acrosin (55, 53, 45, and 38 kDa). All monoclonal antibodies labelled the acrosomes of undamaged spermatozoa in immunofluorescence test. This test should be convenient as an immunological test of the sperm quality.

PMID:: 2073053; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Research Materials

Anti-Acrosin antibody [ACR-2] ab1900 - Abcam plc

Supplemental Content

Save items

Related citations in PubMed

Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction. [Andrologia. 1997]
Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction.
Geussová G, Pĕknicová J, Capková J, Kaláb P, Moos J, Philimonenko VV, Hozák P. Andrologia. 1997 Sep-Oct; 29(5):261-8.
Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro. [Theriogenology. 2001]
Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro.
Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M. Theriogenology. 2001 Jul 15; 56(2):211-23.
Antibody against 28-kDa intra-acrosomal sperm protein as a tool for evaluation of acrosomal integrity in bull spermatozoa. [Folia Biol (Praha). 1998]
Antibody against 28-kDa intra-acrosomal sperm protein as a tool for evaluation of acrosomal integrity in bull spermatozoa.
Peknicová J, Moos J. Folia Biol (Praha). 1998; 44(3):93-6.
Evolution and development of the outer acrosomal membrane (OAM) and evidence that acrosin-inhibitors are proteins of the OAM. [Andrologia. 1987]
Evolution and development of the outer acrosomal membrane (OAM) and evidence that acrosin-inhibitors are proteins of the OAM.
Flörke-Gerloff S, Töpfer-Petersen E, Schill WB, Engel W. Andrologia. 1987 Mar-Apr; 19(2):121-33.
Review Methodological considerations for improving Western blot analysis. [J Pharmacol Toxicol Methods. 2...]
Review Methodological considerations for improving Western blot analysis.
MacPhee DJ. J Pharmacol Toxicol Methods. 2010 Mar-Apr; 61(2):171-7. Epub 2009 Dec 23.

See reviews... See all...

Cited by 4 PubMed Central articles

Effect of estrogens on boar sperm capacitation in vitro. [Reprod Biol Endocrinol. 2010]
Effect of estrogens on boar sperm capacitation in vitro.
Ded L, Dostalova P, Dorosh A, Dvorakova-Hortova K, Peknicova J. Reprod Biol Endocrinol. 2010 Jul 13; 8:87. Epub 2010 Jul 13.
Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study. [Reprod Biol Endocrinol. 2008]
Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study.
Kralova A, Svetlikova M, Madar J, Ulcova-Gallova Z, Bukovsky A, Peknicova J. Reprod Biol Endocrinol. 2008 Jul 2; 6:27. Epub 2008 Jul 2.
Effects of p-nonylphenol and resveratrol on body and organ weight and in vivo fertility of outbred CD-1 mice. [Reprod Biol Endocrinol. 2003]
Effects of p-nonylphenol and resveratrol on body and organ weight and in vivo fertility of outbred CD-1 mice.
Kyselova V, Peknicova J, Buckiova D, Boubelik M. Reprod Biol Endocrinol. 2003 Mar 24; 1:30. Epub 2003 Mar 24.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Monoclonal antibodies against boar acrosomal antigens labelling undamaged acroso...
Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test.
Andrologia. 1990 Sep-Oct ;22(5):427-35.

PubMed
The need for a long-term perspective in child and adolescent psychiatry.
The need for a long-term perspective in child and adolescent psychiatry.
Eur Child Adolesc Psychiatry. 2010 Sep ;19(9):687-8.

PubMed
25-Hydroxyvitamin D in Canadian adults: biological, environmental, and behaviora...
25-Hydroxyvitamin D in Canadian adults: biological, environmental, and behavioral correlates.
Osteoporos Int. 2011 May ;22(5):1389-99. Epub 2010 Aug 21 .

PubMed
Transcriptional regulation by CHIP/LDB complexes.
Transcriptional regulation by CHIP/LDB complexes.
PLoS Genet. 2010 Aug 12 ;6(8):e1001063.

PubMed
[Leiomyoma and leiomyosarcoma of the gastrointestinal system: experience with 5 ...
[Leiomyoma and leiomyosarcoma of the gastrointestinal system: experience with 5 patients and literature review].
Acta Chir Belg. 1990 Sep-Oct ;90(5):213-7.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: