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    Biomaterials. 2010 Nov;31(33):8651-8. Epub 2010 Aug 19.

    Chronic label-free volumetric photoacoustic microscopy of melanoma cells in three-dimensional porous scaffolds.

    Zhang Y, Cai X, Choi SW, Kim C, Wang LV, Xia Y.

    Source

    Department of Biomedical Engineering, Washington University, One Brookings Drive, St Louis, MO 63130, USA.

    Abstract

    Visualizing cells in three-dimensional (3D) scaffolds has been one of the major challenges in tissue engineering. Most current imaging modalities either suffer from poor penetration depth or require exogenous contrast agents. Here, we demonstrate photoacoustic microscopy (PAM) of the spatial distribution and temporal proliferation of cells inside three-dimensional porous scaffolds with thicknesses over 1 mm. Specifically, we evaluated the effects of seeding and culture methods on the spatial distribution of melanoma cells. Spatial distribution of the cells in the scaffold was well-resolved in PAM images. Moreover, the number of cells in the scaffold was quantitatively measured from the as-obtained volumetric information. The cell proliferation profile obtained from PAM correlated well with what was obtained using the traditional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

    Copyright © 2010 Elsevier Ltd. All rights reserved.

    PMID:
    20727581
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC2949455
    Free PMC Article

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