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Bioresour Technol. 2010 Dec;101(24):9815-8. doi: 10.1016/j.biortech.2010.07.078. Epub 2010 Jul 24.

Development of the cellulolytic fungus Trichoderma reesei strain with enhanced beta-glucosidase and filter paper activity using strong artificial cellobiohydrolase 1 promoter.

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  • 1State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.

Abstract

To improve the beta-glucosidase yield and total cellulase activity of Trichoderma reesei, extracellular beta-glucosidase (BGLI) was overexpressed under the control of the modified four-copy cbh1 promoter. Three transformants B2, B12 and B15 with successful integration of the bgl1 gene expression cassette were obtained, which exhibited 3.7-, 2.0- and 1.8-fold increase in beta-glucosidase activity than the parental strain RUT C30, respectively. The filter paper activities of the productive transformants B12 and B15 were improved by up to 130% and 55%, respectively. Saccharification of corncob residue with the crude enzyme dosages showed that the reducing sugar yields of B12 (5.59 mg/ml) and B15 (4.80 mg/ml) were 29% and 11% higher than that of RUT C30 (4.34 mg/ml), respectively. The present results proved that the modified four-copy cbh1 promoter was a useful tool for improving the cellulase activity of T. reesei, and the engineering strains developed in this study could be potentially used as promising cell factories for beta-glucosidase or cellulase production.

Copyright (c) 2010 Elsevier Ltd. All rights reserved.

PMID:
20708927
[PubMed - indexed for MEDLINE]
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