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Am J Obstet Gynecol. 2010 Oct;203(4):402.e1-4. doi: 10.1016/j.ajog.2010.06.060. Epub 2010 Aug 12.

Preimplantation factor promotes first trimester trophoblast invasion.

Author information

  • 1Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale Women and Children's Center for Blood Disorders, New Haven, CT 06520-8063, USA. christina.duzyj@yale.edu

Abstract

OBJECTIVE:

Preimplantation factor is a novel embryo-derived peptide that influences key processes in early pregnancy implantation, including immunity, adhesion, remodeling, and apoptosis. Herein, we explore the effects of synthetic preimplantation factor on trophoblast invasion.

STUDY DESIGN:

Invasion patterns of immortalized cultured HTR-8 trophoblast cells were analyzed through Matrigel extracellular matrix ± synthetic preimplantation factor (25-100 nM) in a transwell assay. Effects were compared with epidermal growth factor 10 μg/mL, scrambled aminoacid sequence of preimplantation factor, or media alone as controls.

RESULTS:

Synthetic preimplantation factor enhances trophoblast invasion at physiologic doses (at 50 nM, 260%; 95% confidence interval [CI], 174-346%; P = .05; 100 nM ,178%; 95% CI, 170-184%; P < .02), compared with scrambled amnioacid sequence preimplantation factor or control media. Epidermal growth factor added to synthetic preimplantation factor does not further enhance trophoblast invasion (synthetic preimplantation factor 50 nM + epidermal growth factor, 238%; 95% CI, 237-239%; P < .03; synthetic preimplantation factor 100 nM + epidermal growth factor 269%; 95% CI, 265-273%; P < .04).

CONCLUSION:

Preimplantation factor should be further investigated as it shows a potential preventative or therapeutic role for pregnancy complications associated with inadequate trophoblast invasion.

Copyright © 2010 Mosby, Inc. All rights reserved.

PMID:
20708167
[PubMed - indexed for MEDLINE]
PMCID:
PMC2947608
Free PMC Article

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