Aberration compensation in confocal microscopy

Appl Opt. 1991 Sep 1;30(25):3563-8. doi: 10.1364/AO.30.003563.

Abstract

In confocal microscopy, spherical aberration is introduced when one is focusing deep within the specimen. This can be compensated for by altering the effective tube length at which the objective is operated. The limitations of this approach are investigated.