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J Biol Chem. 2010 Oct 22;285(43):32778-86. doi: 10.1074/jbc.M110.145094. Epub 2010 Aug 9.

Kinetics of re-establishing H3K79 methylation marks in global human chromatin.

Author information

  • 1Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Abstract

We employ a stable isotope strategy wherein both histones and their methylations are labeled in synchronized human cells. This allows us to differentiate between old and new methylations on pre-existing versus newly synthesized histones. The strategy is implemented on K79 methylation in an isoform-specific manner for histones H3.1, H3.2, and H3.3. Although levels of H3.3K79 monomethylation are higher than that of H3.2/H3.1, the rate of establishing the K79 methylation is the same for all three isoforms. Surprisingly, we find that pre-existing "old" histones continue to be K79-monomethylated and -dimethylated at a rate equal to the newly synthesized histones. These observations imply that some degree of positional "scrambling" of K79 methylation occurs through the cell cycle.

PMID:
20699226
[PubMed - indexed for MEDLINE]
PMCID:
PMC2963384
Free PMC Article

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