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FEBS J. 2010 Sep;277(18):3726-34. doi: 10.1111/j.1742-4658.2010.07773.x. Epub 2010 Aug 5.

MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor.

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  • 1Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.

Abstract

MicroRNAs are an evolutionarily conserved class of endogenous noncoding RNAs that modulate gene expression at the post-transcriptional level. Recently, microRNA-23a (miR-23a) has been found to function as a growth-promoting and antiapoptotic factor in hepatocellular carcinoma cells. Our previous study showed that miR-23a was significantly upregulated in gastric adenocarcinoma tissues. In this study, we found that miR-23a promoted the proliferative potential of gastric adenocarcinoma cell line MGC803. We also identified IL6R as a direct target gene for miR-23a using a fluorescent reporter assay. The mRNA and protein levels of IL6R were both inversely correlated with the miR-23a expression level. Our results demonstrate that miR-23a can target IL6R and promote the growth activity of gastric adenocarcinoma cells in vitro. The downregulation of IL6R by miR-23a may explain why the suppression of miR-23a can inhibit gastric cancer cell proliferation.

© 2010 The Authors Journal compilation © 2010 FEBS.

PMID:
20698883
[PubMed - indexed for MEDLINE]
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