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Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Aug 1;66(Pt 8):909-12. doi: 10.1107/S1744309110020518. Epub 2010 Jul 27.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SAICAR synthase from Streptococcus suis serotype 2.

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  • 1Key Laboratory of Environment Veterinary Science, Shanxi Agricultural University, Shanxi, People's Republic of China.

Abstract

Phosphoribosylaminoimidazole-succinocarboxamide synthase (SAICAR synthase) plays an essential role in the de novo biosynthesis of purine nucleotides. In this study, the SAICAR synthase from Streptococcus suis was cloned and overexpressed in Escherichia coli. The subsequent product was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.8 A resolution and belonged to space group P2, with unit-cell parameters a=70.2, b=52.2, c=153.9 A, beta=102.8 degrees.

PMID:
20693665
[PubMed - indexed for MEDLINE]
PMCID:
PMC2917288
Free PMC Article

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