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Transplant Proc. 2010 Jul-Aug;42(6):2084-6. doi: 10.1016/j.transproceed.2010.05.098.

Comparison of fresh and cultured islets from human and porcine pancreata.

Author information

  • 1Baylor All Saints Medical Center, Baylor Research Institute, Fort Worth, Texas 76104, USA. hirofumn@baylorhealth.edu

Abstract

INTRODUCTION:

For clinical islet transplantation, many centers have recently introduced of human islet cultures prior to transplantation. They provide flexibility to evaluate isolated islets and pretreat patients. However, isolated islets deteriorate rapidly in culture. In the present study, we compared fresh human and porcine islets with cultured islets for c-Jun NH(2)-terminal kinase (JNK) activity.

MATERIALS AND METHODS:

Islet isolations from human and porcine pancreata were performed using the standard Ricordi technique with a modified Edmonton protocol. Isolated islets cultured for 24 hours at 37 degrees C with 5% CO(2) in culture medium were evaluated for counts and JNK activity.

RESULTS:

After 24 hours of culture, the percentages of surviving islets were 86.9% for human and 47.3% for porcine sources. JNK activity in isolated islets declined to a low baseline level after 24-hour culture.

CONCLUSION:

Both human and porcine islets deteriorated rapidly in 24-hour cultures, although the in vitro conditions did not induce JNK activation.

Copyright 2010 Elsevier Inc. All rights reserved.

PMID:
20692414
[PubMed - indexed for MEDLINE]
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