(a) Transmission electron micrograph of Mimivirus. (b) Atomic force microscopy of defibered Mimivirus. The unique star-faced vertex is clearly visible. (c) Fivefold averaged cryo-electron micrographs of virus PBCV-1 reveal a long, thin, cylindrical spike structure at one vertex and protrusions (fibers) extending from one unique capsomer per trisymmetron. (d) Central cross section of panel c. Note the gap between the unique vertex and the membrane enclosing the DNA. Also the unique vertex contains a portal-like protein. (e) PBCV-1 attached to the cell wall as viewed by the quick-freeze, deep-etch procedure. Note fibers attach the virus to the wall. ( f ) Transmission electron micrograph of EhV. (g) Schematic of freshly isolated EhV (left) and stored EhV (right). Note the external membrane swells with age. (h, i) Morphology of the White spot shrimp virus (WSSV) virion. (h) Negative contrast electron micrograph of intact WSSV virion with its tail-like extension. (i ) Schematic based on panel h showing the layered structures of a WSSV virion, i.e., envelope, tegument, and nucleocapsid. ( j ) Electron micrograph of bacteriophage G. The insert shows coliphage lambda to the same scale. Panel a from Reference 10, b from Reference 72, c and d from Reference 7, e from Reference 61, f and g from Reference 36, h and i from Reference 35, and j from Reference 24—all published with permission.