DNA microarray analysis of signaling pathway in macrophages stimulated by lignin-carbohydrate complex from lentinus edodes mycelia (LEM) extract

Anticancer Res. 2010 Jul;30(7):2567-76.

Abstract

In order to investigate the action point of lignin-carbohydrate complex (Fr4) from Lentinus edodes mycelia extract, DNA microarray analysis was performed, using mouse macrophage-like J774.1 cells. Among seven lignin-carbohydrate complex fractions, Fr4 showed the highest stimulatory activity of tumor necrosis factor production by mouse macrophage-like J774.1 cells, as well as its previously reported anti-HIV activity. Fr4 is composed of lignin precursors such as vanillic acid, syringic acid, p-coumaric acid and ferulic acid, with trace amounts of flavonoids and tannins, and negligible amount of lipopolysaccharides (LPS), confirming the authenticity of Fr4 as a lignin. DNA microarray analysis suggested that Fr4 may affect immune response-related gene expression; however, it may not affect the expression of as many genes as LPS does.

MeSH terms

  • Animals
  • Cell Line
  • Cluster Analysis
  • Gene Expression / drug effects
  • Lignin / analysis
  • Lignin / isolation & purification
  • Lignin / pharmacology*
  • Lipopolysaccharides / analysis
  • Lipopolysaccharides / pharmacology
  • Macrophages / drug effects*
  • Macrophages / immunology
  • Macrophages / metabolism
  • Mice
  • Oligonucleotide Array Sequence Analysis / methods
  • Principal Component Analysis
  • Shiitake Mushrooms / chemistry*
  • Signal Transduction / drug effects
  • Stimulation, Chemical
  • Tumor Necrosis Factor-alpha / biosynthesis

Substances

  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha
  • Lignin