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Nano Lett. 2010 Sep 8;10(9):3684-91. doi: 10.1021/nl102180s.

Role of endosomal escape for disulfide-based drug delivery from colloidal mesoporous silica evaluated by live-cell imaging.

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  • 1Department of Chemistry and Center for Nanoscience, University of Munich LMU, Butenandtstrasse 11, Gerhardt-Ertl-Building, 81377 Munich, Germany.

Abstract

Redox-driven intracellular disulfide-cleavage is a promising strategy to achieve stimuli-responsive and controlled drug release. We synthesized colloidal mesoporous silica (CMS) nanoparticles with ATTO633-labeled cysteine linked to the inner particle core via disulfide-bridges and characterized their cysteine release behavior after internalization into HuH7 cells by high-resolution fluorescence microscopy. Our study revealed that endosomal escape is a bottleneck for disulfide-linkage based drug release. Photochemical opening of the endosome leads to successful delivery of fluorescently labeled cysteine to the cytosol.

PMID:
20677799
[PubMed - indexed for MEDLINE]
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