Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Indian Assoc Pediatr Surg. 2009 Apr;14(2):59-62. doi: 10.4103/0971-9261.55153.

Hirschsprung's disease diagnosis: Comparison of immunohistochemical, hematoxilin and eosin staining.

Author information

  • 1Department of Surgery, Medical School, Isfahan University of Medical Sciences.

Abstract

BACKGROUND:

The diagnosis of Hirschsprung's disease (HD) is based on the absence of ganglion cells. In hemotoxilin and eosin (H and E) as well as acetylcholine esterase staining there are limitations in the diagnosis of immature ganglion cells in neonates.

METHODS:

In this prospective study, 54 biopsies taken from suspected HD patients (five mucosal specimens and 49 full thickness specimens) were studied. In the laboratory, after preparing sections of paraffin embedded tissues, H and E staining slides were compared with immunohistochemical (IHC) staining including: S100, NSE, CD117, CD56, Cathepsin D, Vimentin, BCL2, GFAP, Synaptophysin and chromogranin.

RESULTS:

The study revealed 30 negative (absence of ganglion cells) cases (55.5%), 17 positive cases (31.04%) and seven suspected cases (12.9%) of ganglion cells on the H and E staining. On IHC staining with CD56 and Cathepsin D, all of the 17 positive cases detected through H and E, were confirmed for having ganglion cells and out of 30 cases reported negative on H and E staining, 28(93.3%) were reported negative and two (6.7%) positive by IHC staining. Of the seven suspected cases H and E staining), IHC staining detectedganglion cells only in five slides; two remained negative.

CONCLUSIONS:

IHC staining using CD56 and Cathepsin D improved the accuracy of diagnosis in HD when used in addition to H and E staining technique, especially for negative or suspicious slides.

KEYWORDS:

Hirschprung's Disease; immunohistochemical staining; megacolon

PMID:
20671847
[PubMed]
PMCID:
PMC2905532
Free PMC Article

Images from this publication.See all images (4)Free text

Figure 1
Figure 2
Figure 3A
Figure 3B
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
    Loading ...
    Write to the Help Desk