Send to:

Choose Destination
See comment in PubMed Commons below
Pharmacogn Mag. 2010 Apr;6(22):111-5. doi: 10.4103/0973-1296.62896. Epub 2010 May 5.

Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD.

Author information

  • 1Department of Biomaterials Engineering, School of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, Korea.


A HPLC-DAD method was established for the simultaneous evaluation of five bioactive compounds in Ssanghwa tang (SHT) including glycyrrhizin, paeoniflorin, cinnamic acid, decursin and 6-gingerol. These compounds were separated in less than 40 min using a Dionex C(18) column with a gradient elution system of water and methanol at a flow rate of 1 ml/min. Calibration curve of standard components presented excellent linear regression (R(2) > 0.9903) within the test range. Limit of detection and limit of quantification varied from 0.07 to 0.46 mug/ml and 0.13 to 1.11 mug/ml, respectively. The relative standard deviations (RSDs) of data of the intraday and interday experiments were less than 3.67 and 5.73%, respectively. The accuracy of recovery test ranged from 95.98 to 105.88% with RSD values 0.10- 4.82%.


HPLC- DAD; Herbal medicine; Ssanghwa tang; quality control; quantification

Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
    Loading ...
    Write to the Help Desk