Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Pharmacogn Mag. 2010 Apr;6(22):111-5. doi: 10.4103/0973-1296.62896. Epub 2010 May 5.

Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD.

Author information

  • 1Department of Biomaterials Engineering, School of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, Korea.

Abstract

A HPLC-DAD method was established for the simultaneous evaluation of five bioactive compounds in Ssanghwa tang (SHT) including glycyrrhizin, paeoniflorin, cinnamic acid, decursin and 6-gingerol. These compounds were separated in less than 40 min using a Dionex C(18) column with a gradient elution system of water and methanol at a flow rate of 1 ml/min. Calibration curve of standard components presented excellent linear regression (R(2) > 0.9903) within the test range. Limit of detection and limit of quantification varied from 0.07 to 0.46 mug/ml and 0.13 to 1.11 mug/ml, respectively. The relative standard deviations (RSDs) of data of the intraday and interday experiments were less than 3.67 and 5.73%, respectively. The accuracy of recovery test ranged from 95.98 to 105.88% with RSD values 0.10- 4.82%.

KEYWORDS:

HPLC- DAD; Herbal medicine; Ssanghwa tang; quality control; quantification

PMID:
20668576
[PubMed]
PMCID:
PMC2900057
Free PMC Article

Images from this publication.See all images (2)Free text

Figure 1
Figure 2
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Medknow Publications and Media Pvt Ltd Icon for PubMed Central
    Loading ...
    Write to the Help Desk