Format

Send to

Choose Destination
See comment in PubMed Commons below
ACS Appl Mater Interfaces. 2010 Aug;2(8):2465-70. doi: 10.1021/am100534h.

High-sensitivity and stable cellular fluorescence imaging by patterned silver nanocap arrays.

Author information

  • 1Department of Physics, Southeast University, Nanjing 211189, China. tqiu@seu.edu.cn

Abstract

Patterned silver nanocap arrays (PSNAs) prepared on porous anodic alumina templates by a simple coating technique yield enhanced sensitivity and stability in cellular fluorescence imaging. Microstructural analysis, surface-enhanced Raman scattering mapping, and finite difference time domain simulation indicate that the hot spots are evenly distributed on the substrate. Ag1522 or Chinese Hamster Ovary cells are labeled by phalloidin-fluorscein isothiocyanate (P-FITC) on the cytoskeletons and the fluorescence signals from the fluorophores bound on the cell cytoskeletons on the PSNAs are enhanced 8-fold compared to those on glass used in conventional imaging. In addition to the intensity enhancement, the photostability is improved dramatically. Spectral analysis suggests that the PSNAs can create more excitons in the light-emitting P-FITC because of plasmon resonance energy transfer from the silver nanocaps to the nearby P-FITC. They can also act as plasmonic antennae by converting a part of the nonradiative near-field emission from the fluorophores to the far field consequently enhancing the emission.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Write to the Help Desk