Warning: The NCBI web site requires JavaScript to function. more...

My NCBI Sign In

Result Filters

Virology. 2010 Sep 30;405(2):513-23. Epub 2010 Jul 21.

Structure-guided design and immunological characterization of immunogens presenting the HIV-1 gp120 V3 loop on a CTB scaffold.

Totrov M, Jiang X, Kong XP, Cohen S, Krachmarov C, Salomon A, Williams C, Seaman MS, Abagyan R, Cardozo T, Gorny MK, Wang S, Lu S, Pinter A, Zolla-Pazner S.

Source

Molsoft LLC, 3366 N Torrey Pines Ct., La Jolla, CA 92037, USA. max@molsoft.com

Erratum in

Virology. 2011 Jan 20;409(2):360. Abagyan, Ruben [added].

Abstract

V3 loop is a major neutralizing determinant of the HIV-1 gp120. Using 3D structures of cholera toxin B subunit (CTB), complete V3 in the gp120 context, and V3 bound to a monoclonal antibody (mAb), we designed two V3-scaffold immunogen constructs (V3-CTB). The full-length V3-CTB presenting the complete V3 in a structural context mimicking gp120 was recognized by the large majority of our panel of 24 mAbs. The short V3-CTB presenting a V3 fragment in the conformation observed in the complex with the 447-52D Fab, exhibited high-affinity binding to this mAb. The immunogens were evaluated in rabbits using DNA-prime/protein-boost protocol. Boosting with the full-length V3-CTB induced high anti-V3 titers in sera that potently neutralize multiple HIV virus strains. The short V3-CTB was ineffective. The results suggest that very narrow antigenic profile of an immunogen is associated with poor Ab response. An immunogen with broader antigenic activity elicits robust Ab response.

Copyright 2010 Elsevier Inc. All rights reserved.

PMID:: 20663531; [PubMed - indexed for MEDLINE]
PMCID:: PMC2942080

Free PMC Article

Images from this publication.See all images (6) Free text

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical

HIV InSite

Supplemental Content

Icon for Elsevier Science

Icon for PubMed Central

Save items

loading

Related citations in PubMed

Design of immunogens that present the crown of the HIV-1 V3 loop in a conformation competent to generate 447-52D-like antibodies. [Biochem J. 2006]
Design of immunogens that present the crown of the HIV-1 V3 loop in a conformation competent to generate 447-52D-like antibodies.
Chakraborty K, Durani V, Miranda ER, Citron M, Liang X, Schleif W, Joyce JG, Varadarajan R. Biochem J. 2006 Nov 1; 399(3):483-91.
Cross-clade HIV-1 neutralizing antibodies induced with V3-scaffold protein immunogens following priming with gp120 DNA. [J Virol. 2011]
Cross-clade HIV-1 neutralizing antibodies induced with V3-scaffold protein immunogens following priming with gp120 DNA.
Zolla-Pazner S, Kong XP, Jiang X, Cardozo T, Nádas A, Cohen S, Totrov M, Seaman MS, Wang S, Lu S. J Virol. 2011 Oct; 85(19):9887-98. Epub 2011 Jul 27.
Mapping the immune response to the outer domain of a human immunodeficiency virus-1 clade C gp120. [J Gen Virol. 2008]
Mapping the immune response to the outer domain of a human immunodeficiency virus-1 clade C gp120.
Chen H, Xu X, Lin HH, Chen SH, Forsman A, Aasa-Chapman M, Jones IM. J Gen Virol. 2008 Oct; 89(Pt 10):2597-604.
Characterization of an internal permissive site in the cholera toxin B-subunit and insertion of epitopes from human immunodeficiency virus-1, hepatitis B virus and enterotoxigenic Escherichia coli. [Gene. 1995]
Characterization of an internal permissive site in the cholera toxin B-subunit and insertion of epitopes from human immunodeficiency virus-1, hepatitis B virus and enterotoxigenic Escherichia coli.
Bckström M, Holmgren J, Schödel F, Lebens M. Gene. 1995 Nov 20; 165(2):163-71.
Review Structural studies of human HIV-1 V3 antibodies. [Hum Antibodies. 2005]
Review Structural studies of human HIV-1 V3 antibodies.
Stanfield RL, Wilson IA. Hum Antibodies. 2005; 14(3-4):73-80.

See reviews... See all...

Cited by 4 PubMed Central articles

Co-immunization with multimeric scaffolds and DNA rapidly induces potent autologous HIV-1 neutralizing antibodies and CD8+ T cells. [PLoS One. 2012]
Co-immunization with multimeric scaffolds and DNA rapidly induces potent autologous HIV-1 neutralizing antibodies and CD8+ T cells.
Jaworski JP, Krebs SJ, Trovato M, Kovarik DN, Brower Z, Sutton WF, Waagmeester G, Sartorius R, D'Apice L, Caivano A, et al. PLoS One. 2012; 7(2):e31464. Epub 2012 Feb 16.
Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs. [Adv Virol. 2011]
Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs.
Challa S, Szczepanek SM, Rood D, Barrette RW, Silbart LK. Adv Virol. 2011; 2011:713769. Epub 2011 Sep 22.
Can HIV p24 be a suitable scaffold for presenting Env antigens? [Clin Vaccine Immunol. 2011]
Can HIV p24 be a suitable scaffold for presenting Env antigens?
Buonaguro L, Tagliamonte M, Tornesello ML, Buonaguro FM. Clin Vaccine Immunol. 2011 Nov; 18(11):2003-4. Epub 2011 Sep 7.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Structure-guided design and immunological characterization of immunogens present...
Structure-guided design and immunological characterization of immunogens presenting the HIV-1 gp120 V3 loop on a CTB scaffold.
Virology. 2010 Sep 30 ;405(2):513-23. Epub 2010 Jul 21 .

PubMed
Fish meal as a protein supplement in ruminant diets: a review.
Fish meal as a protein supplement in ruminant diets: a review.
J Anim Sci. 1991 May ;69(5):2147-56.

PubMed
Perceptions and behaviors related to hand hygiene for the prevention of H1N1 inf...
Perceptions and behaviors related to hand hygiene for the prevention of H1N1 influenza transmission among Korean university students during the peak pandemic period.
BMC Infect Dis. 2010 Jul 28 ;10:222.

PubMed
Increased expression of cardiac IL-17 after burn.
Increased expression of cardiac IL-17 after burn.
J Inflamm (Lond). 2010 Jul 27 ;7:38.

PubMed
Comparison of the auditory sensitivity of neurons in the cochlear nucleus and in...
Comparison of the auditory sensitivity of neurons in the cochlear nucleus and inferior colliculus of young and aging C57BL/6J and CBA/J mice.
Hear Res. 1991 May ;53(1):78-94.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...

You are here: NCBI > Literature > PubMed

Write to the Help Desk