Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
PLoS One. 2010 Jul 16;5(7):e11621. doi: 10.1371/journal.pone.0011621.

A Phos-tag-based approach reveals the extent of physiological endoplasmic reticulum stress.

Author information

  • 1Graduate Program in Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York, United States of America.

Abstract

Cellular response to endoplasmic reticulum (ER) stress or unfolded protein response (UPR) is a key defense mechanism associated with many human diseases. Despite its basic and clinical importance, the extent of ER stress inflicted by physiological and pathophysiological conditions remains difficult to quantitate, posing a huge obstacle that has hindered our further understanding of physiological UPR and its future therapeutic potential. Here we have optimized a Phos-tag-based system to detect the activation status of two proximal UPR sensors at the ER membrane. This method allowed for a quantitative assessment of the level of stress in the ER. Our data revealed quantitatively the extent of tissue-specific basal ER stress as well as ER stress caused by the accumulation of misfolded proteins and the fasting-refeeding cycle. Our study may pave the foundation for future studies on physiological UPR, aid in the diagnosis of ER-associated diseases and improve and facilitate therapeutic strategies targeting UPR in vivo.

PMID:
20661282
[PubMed - indexed for MEDLINE]
PMCID:
PMC2905412
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk