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Genetics. 2010 Oct;186(2):757-61. doi: 10.1534/genetics.110.120717. Epub 2010 Jul 26.

Targeting DNA double-strand breaks with TAL effector nucleases.

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  • 1Department of Plant Pathology, Iowa State University, Ames, Iowa 50011, USA.


Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.

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