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J Cell Biol. 2010 Jul 26;190(2):165-75. doi: 10.1083/jcb.201002018. Epub 2010 Jul 19.

A guide to super-resolution fluorescence microscopy.

Author information

  • 1Department of Biology and Center for Integrated Protein Science, Ludwig Maximilians University Munich, 82152 Planegg-Martinsried, Germany. lothar.schermelleh@lmu.de

Abstract

For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new approaches have created unprecedented new possibilities to investigate the structure and function of cells.

PMID:
20643879
[PubMed - indexed for MEDLINE]
PMCID:
PMC2918923
Free PMC Article

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