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Nat Methods. 2010 Aug;7(8):619-21. doi: 10.1038/nmeth.1480. Epub 2010 Jul 18.

Amplification-free digital gene expression profiling from minute cell quantities.

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  • 1Helicos BioSciences Corporation, Cambridge, Massachusetts, USA. fatihozsolak@gmail.com

Abstract

Generating reliable expression profiles from minute cell quantities is critical for scientific discovery and potential clinical applications. Here we present low-quantity digital gene expression (LQ-DGE), an amplification-free approach involving capture of poly(A)(+) RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface reverse transcription and sequencing. We applied LQ-DGE to profile malignant and nonmalignant mouse and human cells, demonstrating its quantitative power and potential applicability to archival specimens.

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PMID:
20639869
[PubMed - indexed for MEDLINE]
PMCID:
PMC2975601
Free PMC Article

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