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Nat Biotechnol. 2010 Aug;28(8):856-62. doi: 10.1038/nbt.1653. Epub 2010 Jul 18.

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides.

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  • 1Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, USA.

Abstract

A fundamental goal in biotechnology and biology is the development of approaches to better understand the genetic basis of traits. Here we report a versatile method, trackable multiplex recombineering (TRMR), whereby thousands of specific genetic modifications are created and evaluated simultaneously. To demonstrate TRMR, in a single day we modified the expression of >95% of the genes in Escherichia coli by inserting synthetic DNA cassettes and molecular barcodes upstream of each gene. Barcode sequences and microarrays were then used to quantify population dynamics. Within a week we mapped thousands of genes that affect E. coli growth in various media (rich, minimal and cellulosic hydrolysate) and in the presence of several growth inhibitors (beta-glucoside, D-fucose, valine and methylglyoxal). This approach can be applied to a broad range of traits to identify targets for future genome-engineering endeavors.

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PMID:
20639866
[PubMed - indexed for MEDLINE]
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