Format

Send to

Choose Destination
See comment in PubMed Commons below
J Neuroimmunol. 2010 Oct 8;227(1-2):167-74. doi: 10.1016/j.jneuroim.2010.06.010. Epub 2010 Jul 31.

Measurement of anti-beta amyloid antibodies in human blood.

Author information

  • 1Department of Neurology and Neurosciences, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA.

Abstract

The human IgG repertoire contains endogenous antibodies against beta amyloid (Aβ) that may be relevant to the pathogenesis and treatment of Alzheimer's disease. There have been widely disparate estimates of the levels of these antibodies in human plasma. We identify factors that have contributed to these disparities and describe improved methods for measuring anti-Aβ antibodies in blood. These methods include isolating immunoglobulin by thiophilic chromatography and using chaotropic salts to dislodge weakly bound antibodies without significantly reducing the binding of specific anti-Aβ antibodies. Using these methods, we show that human blood contains polyvalent IgG antibodies that bind to Aβ with relatively low avidity and specificity, as well as IgG antibodies that bind to linear and conformational epitopes on amyloid monomers and aggregates with moderate to high avidity.

Copyright © 2010 Elsevier B.V. All rights reserved.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk