Induction of Th1 immune responses to Japanese cedar pollen allergen (Cry j 1) in mice immunized with Cry j 1 conjugated with CpG oligodeoxynucleotide

Comp Immunol Microbiol Infect Dis. 2011 Mar;34(2):157-61. doi: 10.1016/j.cimid.2010.06.005. Epub 2010 Jul 17.

Abstract

We determined whether a major Japanese cedar pollen allergen (Cry j 1) conjugated with CpG oligodeoxynucleotide would enhance allergen-specific Th1 responses in mice. Cry j 1 conjugated with CpG (Cry j 1-CpG) induced IL-12 in the spleen cells of naïve mice. Cry j 1-CpG immunization of BALB/c mice suppressed anti-Cry j 1 IgE response and enhanced anti-Cry j 1 IgG(2a) to subsequent Cry j 1 and alum adjuvant injection. CD4(+)T cells isolated from the spleens in mice immunized with Cry j 1-CpG produced higher IFN-γ levels than did CD4(+)T cells obtained from mice as negative controls. Our results suggested that Cry j 1-CpG immunization can induce Cry j 1-specific Th1 immune responses, thereby inhibiting IgE response to the pollen allergen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Plant / immunology*
  • Cross Reactions
  • Female
  • Immunization
  • Immunoglobulin E / immunology
  • Immunoglobulin G / immunology
  • Interleukin-12 Subunit p40 / immunology
  • Mice
  • Mice, Inbred BALB C
  • Oligodeoxyribonucleotides / immunology*
  • Plant Proteins / immunology*
  • Spleen / immunology
  • Th1 Cells / immunology*
  • Th2 Cells / immunology

Substances

  • Antigens, Plant
  • CPG-oligonucleotide
  • Cry j I protein, Cryptomeria japonica
  • Immunoglobulin G
  • Interleukin-12 Subunit p40
  • Oligodeoxyribonucleotides
  • Plant Proteins
  • Immunoglobulin E