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BMC Cancer. 2010 Jul 19;10:376. doi: 10.1186/1471-2407-10-376.

Relationship between EGFR expression, copy number and mutation in lung adenocarcinomas.

Author information

  • 1Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijng 100730, China.

Abstract

BACKGROUND:

This study was designed to investigate EGFR protein expression, EGFR copy number and EGFR mutations in lung adenocarcinomas, to explore the relationship of the three markers.

METHODS:

EGFR status was analyzed in surgically resected lung adenocarcinoma samples from 133 Chinese patients by three methods: protein expression (n=133) by standardized immunohistochemistry (IHC), gene copy number (n=133) by fluorescence in situ hybridization (FISH), and mutation analysis using the Scorpion amplification refractory mutation system (ARMS) (n=133).

RESULTS:

The results showed that 68.4% of the samples were positive by IHC, 42.1% were positive by FISH, and 63.9% contained activating kinase domain mutations. EGFR mutations were more frequent in non-smoking patients (p=0.008), and EGFR mutations were associated with EGFR FISH positivity (p<0.0001). When using 10% positivity and 2+ as cutoffs, EGFR protein expression was significantly correlated with EGFR FISH positivity (p=0.012) and EGFR mutations (p=0.008) after Bonferroni correction.

CONCLUSION:

EGFR protein expression, EGFR copy number and EGFR mutations were closely related to each other. Standard methods and interpretation criteria need to be established.

PMID:
20637128
[PubMed - indexed for MEDLINE]
PMCID:
PMC2913962
Free PMC Article
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