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Fertil Steril. 2010 Dec;94(7):2528-30. doi: 10.1016/j.fertnstert.2010.04.054.

Detection of mitochondrial biomarkers in eutopic endometria of endometriosis using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry.

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  • 1Department of Gynecology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.

Abstract

OBJECTIVES:

To detect specific mitochondrial proteins in eutopic endometrial samples from women with and without endometriosis and to build diagnostic models.

DESIGN:

Eutopic endometrial samples from women with endometriosis (excluding adenomyosis) and women with benign indications as control were studied by using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein-chip technology. After finding the biomarkers, the diagnostic model was evaluated and validated by leave-one cross-validation.

SETTING:

Collaborative investigation in an academic research environment.

PATIENT(S):

Twenty-four patients with endometriosis (excluding adenomyosis) and 29 patients with benign indications as control.

INTERVENTION(S):

Surgical excision of eutopic endometrial biopsy of patients with endometriosis and controls.

MAIN OUTCOME MEASURE(S):

Mitochondrial protein expression.

RESULT(S):

Seventy-eight qualified mitochondrial protein peaks were detected, ten of them had a significant difference. Three combined potential biomarkers, with mass-to-charge ratios (m/z) of 15,334, 15,128, and 16,069, were found, and the diagnostic system distinguished endometriosis from control samples with a specificity of 86.2% and a sensitivity of 87.5%.

CONCLUSION(S):

We discovered potential mitochondrial biomarkers of eutopic endometrium in endometriosis and set up a diagnostic model. Further identification of the proteins we found will help to explain pathology, new diagnoses, and therapeutic approaches for endometriosis.

Crown Copyright © 2010. Published by Elsevier Inc. All rights reserved.

PMID:
20627238
[PubMed - indexed for MEDLINE]
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