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Methods Enzymol. 2010;475:321-75. doi: 10.1016/S0076-6879(10)75014-X.

Single-molecule dual-beam optical trap analysis of protein structure and function.

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  • 1Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA.

Abstract

Optical trapping is one of the most powerful single-molecule techniques. We provide a practical guide to set up and use an optical trap, applied to the molecular motor myosin as an example. We focus primarily on studies of myosin function using a dual-beam optical trap, a protocol to build such a trap, and the experimental and data analysis protocols to utilize it.

Copyright (c) 2010 Elsevier Inc. All rights reserved.

PMID:
20627164
[PubMed - indexed for MEDLINE]
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