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Mol Cell. 2010 Jun 25;38(6):815-26. doi: 10.1016/j.molcel.2010.05.022.

The activation potential of MOF is constrained for dosage compensation.

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  • 1Adolf-Butenandt-Institute and Centre for Integrated Protein Science, Ludwig-Maximilians-University, 80336 Munich, Germany.

Abstract

The H4K16 acetyltransferase MOF plays a crucial role in dosage compensation in Drosophila but has additional, global functions. We compared the molecular context and effect of MOF in male and female flies, combining chromosome-wide mapping and transcriptome studies with analyses of defined reporter loci in transgenic flies. MOF distributes dynamically between two complexes, the dosage compensation complex and a complex containing MBD-R2, a global facilitator of transcription. These different targeting principles define the distribution of MOF between the X chromosome and autosomes and at transcription units with 5' or 3' enrichment. The male X chromosome differs from all other chromosomes in that H4K16 acetylation levels do not correlate with transcription output. The reconstitution of this phenomenon at a model locus revealed that the activation potential of MOF is constrained in male cells in the context of the DCC to arrive at the 2-fold activation of transcription characteristic of dosage compensation.

Copyright (c) 2010 Elsevier Inc. All rights reserved.

PMID:
20620953
[PubMed - indexed for MEDLINE]
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