(A) Clonogenic survival assay of irradiated cells in methylcellulose (n = 9; ***p ≤ 0.001 [CMPs and GMPs vs. HSPCs]; •p ≤ 0.05 [GMPs vs. CMPs]).
(B) Clonogenic survival assay of irradiated Atm^−/− cells in methylcellulose (n = 3; *p ≤ 0.05 [Atm^+/+ GMPs vs. Atm^+/+ HSPCs]; °°°p ≤ 0.001 [Atm^−/− vs. Atm^+/+ populations]).
(C) Representative example of CFSE dilution assay in unirradiated (grey) or 2Gy-irradiated (color) cells grown for up to 3 days in liquid media (n = 4).
(D) Intracellular cleaved caspase 3 staining in unirradiated (grey) or 2Gy-irradiated WT (left side; solid colors; n = 10) or H2k-bcl2 (right side; striped colors; n = 3) cells grown for up to 2 days in liquid media (p ≤ 0.001, *p ≤ 0.05 [unirradiated vs. irradiated cells]; °°°p ≤ 0.001, °p ≤ 0.05 [H2k-bcl2 vs. WT cells]; ns: not significant).
(E) QRT-PCR analysis of the basal expression level of bcl2-family pro-survival and pro-apoptotic genes, Trp53 and p21 in freshly isolated cells. Results are expressed as log2 fold expression compared to levels measured in HSPCs (n = 6; ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 [CMPs vs. HSPCs]; °°°p ≤ 0.001, °°p ≤ 0.01, °p ≤ 0.05 [GMPs vs. HSPCs]).
(F) Western blot analysis of Mcl-1 and Bid protein levels in purified cells (protein extracted from 35,000 isolated cells per lane; β-actin is used as loading control).
See also Figure S1.

(A) Immunofluorescence microscopy of ionizing radiation-induced foci (IRIF) of γH2AX in unirradiated or 2Gy-irradiated HSPCs (n = 13), CMPs (n = 8) and GMPs (n = 10). The percentage of positive cells (≥6 γH2AX positive foci) is shown over 24 hours (*p ≤ 0.05 [CMPs vs. HSPCs]; °°°p ≤ 0.001 [GMPs vs. HSPCs]; scale bar = 10 μm).
(B) Representative examples of COMET tail DNA content scoring from un-damaged (0), increasingly damaged (1–3) to very damaged (4) cells.
(C) Quantification of tail DNA content scores in unirradiated or 2Gy irradiated HSPCs, CMPs and GMPs after 2 and 24 hours. Results are normalized to the number of cells counted per field (n = 3; **p ≤ 0.01).
See also Table S1.

(A) In vitro 24 hour pre-culture (24hr preC) and in vivo cyclosphosphamide/G-CSF mobilization (Mob.) strategies used to induce proliferation of quiescent (Rest.) HSPCs.
(B) Proliferation rates measured after 1 hour BrdU pulse in vitro (n = 3; •••p ≤ 0.001; [proliferating HSPCs vs. resting HSPCs]).
(C) Quiescence status measured by intracellular 7AAD/Pyronin Y staining.
(D) Clonogenic survival assay in methylcellulose (n = 3).
(E) Growth in liquid media (n = 3).
(G) Intracellular cleaved caspase 3 staining in unirradiated (grey) or 2Gy-irradiated (color) resting and proliferating HSPCs grown for up to 2 days in liquid media (n = 3; ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 [proliferating HSPCs ± IR vs. resting HSPCs ± IR]; ns: not significant).
(F) Example of CFSE dilution assay in unirradiated (grey) or 2Gy-irradiated (color) quiescent and proliferating HSPCs grown for 2 days in liquid media (n = 3).
See also Figure S5.

(A) Summary of the SKY analyses performed on the in vitro progeny of 2Gy-irradiated quiescent HSPCs.
(B) Average number of genomic rearrangement (left side) and percentage (%) of aberrant cells (right side) identified by SKY analysis in the in vitro progeny of 2Gy-irradiated quiescent (Rest.; n = 4) and proliferating (Mob./24h preC; n = 3) HSPCs (*p ≤ 0.05).
(C) Experimental design of the in vivo analysis of 2Gy-irradiated HSPCs assessing long-term reconstitution and genomic instability.
(D) Summary of the SKY analysis performed on the in vivo MP progeny of 2Gy-irradiated quiescent HSPCs 4 months after transplantation.
(E) In cohort I2, 1,500 ±IR HSPC together with 300,000 Sca-1-depleted helper bone marrow cells were transplanted per recipient (n = 5 [0Gy] and 4 [2Gy] mice per group). Long-term reconstitution was measured by sustained CD45.1 chimerism in the peripheral blood of primary transplanted mice (left graph; expressed as percent of the engraftment provided by unirradiated HSPCs) and secondary transplantation of donor-derived HSPCs re-isolated from pooled primary transplanted animals (right graph; expressed as engraftment ratio of CD45.1⁺ cells at 4 months post-transplantation; n = 4 [0Gy] and 5 [2Gy] mice per group).
See also Figure S6, Table S2 and Table S3.

(A) Intracellular FACS analysis of p53 and actin protein levels in unirradiated (-IR) or 2Gy irradiated (+IR) mice 12 hours after exposure.
(B) QRT-PCR analysis of p53 target genes in WT cells 8 and 12 hours after 2Gy IR treatment. Results are expressed as log2 fold expression compared to levels measured in unirradiated cells cultured in the same conditions (n = 3; ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05) or 12 hours (n = 3; °°°p ≤ 0.001, °°p ≤ 0.01, °p ≤ 0.05).
(C) Clonogenic survival assay of irradiated Trp53^−/− cells in methylcellulose (n = 3; **p ≤ 0.01, *p ≤ 0.05 [Trp53^−/− vs. Trp53^+/+ cells]).
(D) Example of CFSE dilution assay in unirradiated (-IR: grey) or 2Gy-irradiated (+IR: blue) Trp53^+/+ (solid) and Trp53^−/− (striped) HSPCs grown for 2 days in liquid media (n = 3).
(E) Intracellular cleaved caspase 3 staining in unirradiated (grey) or 2Gy-irradiated Trp53^+/+ (solid colors) and Trp53^−/− (striped colors) cells grown for up to 2 days in liquid media (n = 3; ***p ≤ 0.001, ** p ≤ 0.01, *p ≤ 0.05 [unirradiated vs. irradiated cells]; °°p ≤ 0.01, °p ≤ 0.05 [Trp53^−/− vs. Trp53^+/+ cells]; ns: not significant).
(F) QRT-PCR analysis of p53-target genes in Trp53^−/− cells 12 hours after 2Gy IR treatment. Results are expressed as log2 fold expression compared to levels measured in unirradiated Trp53^−/− cells cultured in the same conditions (n = 3).
See also Figure S2

(A) Immunofluorescence microscopy of Rad51 IRIF in unirradiated and 2Gy-irradiated HSPCs (n = 5), CMPs (n = 6) and GMPs (n = 8). The percentage of positive cells (≥3 Rad51 positive foci) is shown over 24 hours (***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 [CMPs vs. HSPCs]; °°°p ≤ 0.001, °p ≤ 0.05 [GMPs vs. HSPCs]; scale bar = 10 μm).
(B) Immunofluorescence microscopy of 53BP1 IRIF in unirradiated and 2Gy-irradiated HSPCs (n = 9), CMPs (n = 7) and GMPs (n = 9). The percentage of positive cells (≥3 53BP1 positive foci) is shown over 24 hours (***p ≤ 0.001, *p ≤ 0.05 [CMPs vs. HSPCs]; °°°p ≤ 0.001, °°p ≤ 0.01 [GMPs vs. HSPCs]; scale bar = 10 μm).
(C) QRT-PCR analysis of homologous recombination (HR) and nonhomologous end joining (NHEJ) DNA repair genes in freshly isolated cells. Results are expressed as log2 fold expression compared to levels measured in HSPCs (n = 3; ***p ≤ 0.001, *p ≤ 0.05 [CMPs vs. HSPCs]; °°°p ≤ 0.001, °°p ≤ 0.01, °p ≤ 0.05 [GMPs vs. HSPCs]).
(D) Quantification of NHEJ activity in unirradiated and 2Gy-irradiated cells. Results are expressed as fold changes upon IR-treatment (n = 5; ***p ≤ 0.001, **p ≤ 0.01).
See also Figure S3 and Figure S4.

(A) Immunofluorescence microscopy of Rad51 IRIF in 2Gy-irradiated resting HSPCs (n = 5), 24h pre-cultured HSPCs (n = 3) and mobilized HSPCs (n = 5). The percentage of positive cells (≥ 3 Rad51 positive foci) is shown over 24 hours (***p ≤ 0.001, *p ≤ 0.05 [24h preC. vs. Rest. HSPCs]; °°°p ≤ 0.001 [Mob. vs. Rest. HSPCs]; scale bar = 10 μm).
(B) Immunofluorescence microscopy of 53BP1 IRIF in 2Gy-irradiated resting HSPCs (n = 9), 24h pre-cultured HSPCs (n = 3) and mobilized HSPCs (n = 5). The percentage of positive cells (≥ 3 53BP1 positive foci) is shown over 24 hours (***p ≤ 0.001 [24hr preC. vs. Rest. HSPCs]; °°°p ≤ 0.001, °°p ≤ 0.01 [Mob. vs. Rest. HSPCs]; scale bar = 10 μm).
(C) QRT-PCR analysis of HR and NHEJ repair genes in resting and proliferating HSPCs (n = 3; ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 [24h preC vs. Rest. HSPCs]; °°°p ≤ 0.001, °°p ≤ 0.01, °p ≤ 0.05 [Mob. vs. Rest. HSPCs]).
(D) Quantification of NHEJ activity in unirradiated and 2Gy-irradiated resting and proliferating HSPCs. Results are average ± SEM (error bars) of 2 (24h preC. and Mob. HSPCs) to 5 (Rest. HSPCs) independent experiments and are expressed as fold changes upon IR-treatment (*p 0.05).
See also Figure S4 and Figure S5.